당뇨유도 백서 경골에 티타늄 임플란트 매식 시 지방조직 유래 줄기세포 주입 후 저출력 초음파 적용이 골치유에 미치는 영향
Effect on bone healing by the application of low intensity pulsed ultrasound after injection of adipose tissue-derived stem cells at the implantation of titanium implant in the tibia of diabetes-induced rat
정태영, 박상준, 황대석, 김용덕, 이수운, 김욱규,
소속 상세정보
정태영 ( Jung Tae-Young ) - 인제대학교 의과대학 부산백병원 구강악안면외과
박상준 ( Park Sang-Jun ) - 인제대학교 의과대학 부산백병원 구강악안면외과
황대석 ( Hwang Dae-Seok ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
김용덕 ( Kim Yong-Deok ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
이수운 ( Lee Soo-Woon ) - 인제대학교 해운대백병원 구강악안면외과
김욱규 ( Kim Uk-Kyu ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
KMID : 0355620110370040301
Abstract
Introduction:This study examined the effect of the application of low intensity pulsed ultrasound on bone healing after an injection of adipose tissue-derived stem cells (ADSCs) during the implantation of a titanium implant in the tibia of diabetes-induced rats.
Materials and Methods:: Twelve Sprague-Dawely rats were used. After inducing diabetes, the ADSCs were injected into the hole for the implant. Customized screw type implants, 2.0 ㎜ in diameter and 3.5 ㎜ in length, were implanted in both the tibia of the diabetes-induced rats. After implantation, LIPUS was applied with parameters of 3 ㎒, 40 ㎽/㎠, and 10 minutes for 7 days to the left tibiae (experimental group) of the diabetes-induced rats. The right tibiae in each rat were used in the control group. At 1, 2 and 4 week rats were sacrificed, and the bone tissues of both tibia were harvested. The bone tissues of the three rats in each week were used for bone-to-implant contact (BIC) and bone area (BA) analyses and the bone tissues of one rat were used to make sagittal serial sections.
Results: In histomorphometric analyses, the BIC in the experimental and control group were respectively, 39.00±18.17% and 42.87±9.27% at 1 week, 43.74±6.83% and 32.27±6.00% at 2 weeks, and 32.62±11.02% and 47.10±9.77% at 4 weeks. The BA in experimental and control group were respectively, 37.28±3.68% and 31.90±2.84% at 1 week, 20.62±2.47% and 15.64±2.69% at 2 weeks, and 11.37±4.54% and 17.69±8.77% at 4 weeks. In immunohistochemistry analyses, Osteoprotegerin expression was strong at 1 and 2 weeks in the experimental group than the control group. Receptor activator of nuclear factor kB ligand expression showed similar staining at each week in the experimental and control group.
Conclusion: These results suggest that the application of low intensity pulsed ultrasound after an injection of adipose tissue-derived stem cells during the implantation of titanium implants in the tibia of diabetes-induced rats provided some positive effect on bone regeneration at the early stage after implantation. On the other hand, this method is unable to increase the level of osseointegration and bone regeneration of the implant in an uncontrolled diabetic patient.
키워드
Diabetes; Implant; Low intensity pulsed ultrasound; ADSCs
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